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Construct size (kb): 45.16299819946289

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: svPHEP
Intact vector size: 9.201
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI ClaI
Polylinker sites: HindIII ClaI EcoRI BamHI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L19900

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Genomic copy number: unique
Gene name: gene X
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Genome: Homo sapiens
Gene symbol: ABL1
Other gene symbols: ABL
Genomic copy number: unique
Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Tissue: lung carcinoma
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 35.962
Cross references: DNA Seq. Acc.: U07561
Nucleotides 1-35962 of the insert correspond to
nucleotides 1-35962 of U07561.

Insert lengths(kb): 35.96200180053711

Tissue: lung carcinoma

Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL]

Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1

Restriction digests of the clone give the following sizes (kb): BamHI-->23.0; BglII--12.0, 6.6, 6.1, 5.8, 4.6 (doublet), 4.2, 2.8, 0.52; EcoRI--8.0, 7.0, 6.6, 4.9, 4.2 (doublet), 2.65, 2.15 (doublet), 1.55 (doublet);

HindIII--14.0 (doublet), 6.1, 2.55 (doublet), 2.3, 2.05, 1.15, 0.96, 0.74, 0.44; SstI--14.0, 10.5, 7.0, 5.8 (doublet), 3.8, 0.92.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

The insert begins 8604 nt upstream of "gene X", includes all 8 exons of that gene (through nt 18583) and continues through exon 1b (29132-29267) of ABL1. A CML breakpoint occurs in the intergenic region.