Quality control of chromID Clostridioides difficile agar
Enteric Research
Isolation
Human feces, Albuquerque, NM, USA, 2007
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Genotype
Toxinotype: XXII
Type Strain
no
Comments
Binary Toxin gene cdtB was amplified by PCR
Presence of tcdA and tcdB genes confirmed by PCR.
Ribotype 251
Toxinotype XXII
Medium
ATCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
ATCC® Medium 2107: Modified Reinforced Clostridial
Growth Conditions
Temperature: 37°C
Atmosphere: Anaerobic
Name of Depositor
N Moss
References
Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986
Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Stubbs, S et al. Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile. FEMS Microbiol. Lett. 186(2): 307-312, 2000. PubMed: 10802189
