| 產品名稱 |
pTW11 [CMCC 2210] |
| 商品貨號 |
B210184 |
| Designations |
pTW11 [CMCC 2210] |
| GenBank Number |
M73789
|
| Species |
Streptomyces rubiginosus (Preobrazhenskaya et al.) Pridham et al. |
| Depositors |
Cetus Corp., K Van Note, Cetus Corp. |
| Applications |
produces protein D-xylose isomerase |
| Vector |
Construct size (kb): 5.659999847412109 |
| Insert |
DNA: genomic Insert lengths(kb): 1.200000047683716 Gene product: D-xylose isomerase [xylA] Target Gene: D-xylose isomerase |
| Insert Size (kb) |
1.200 |
| Media |
ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 1.2; SalI--4.8, 0.7; XbaI--uncut. To induce expression of the insert, grow in LB with limiting concentrations of tryptophan. A genomic NruI/SmaI fragment was modified and cloned into pTrp3. Contrary to the description published in J. Bacteriology 173, 6849-6858, 1991, this construct appears to have had HindIII sites engineered up and downstream of the coding region. |
| References |
Drummond RJ, et al. Procaryotic xylose isomerase muteins. US Patent 5,041,378 dated Aug 20 1991
Wong HC, et al. Genetic organization and regulation of the xylose degradationgenes in Streptomyces rubiginosus. J. Bacteriol. 173: 6849-6858, 1991. PubMed: 1657868
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
