| 產(chǎn)品名稱 | Salpingoeca rosetta |
|---|---|
| 商品貨號 | B209708 |
| Deposited As | Proterospongia sp. |
| Strain Designations | BSB-021 |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Type Strain | no |
| Comments | Previously described as a member of the genus
Proterospongia. Renamed as Salpingoeca rosetta based on molecular phylogeny and character trait. Ref   Dayel MJ, et al. Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosetta. Dev. Biol. 357: 73-82, 2011. PubMed: 21699890 |
| Medium | ATCC® Medium 1525: Seawater 802 medium |
| Growth Conditions | Temperature: 25.0°C Growth condition: xenic, with mixed bacteria |
| Cryopreservation | Cryoprotective Solution DMSO ?????????????????????????????????????????????????????????????????????????????????? 2.0 ml Fresh growth medium w/o bacteria???????????????????????????????? 8.0 ml 1.???? Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat. 2. ?? Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min. 3. ??? Adjust the concentration of cells to at least 2 x 106/ml in fresh medium. 4.? ?? Mix the cell preparation and the cryoprotective solution in equal portions. 5.? ?? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). 6. ??? Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately -1°C/min.) ? 7.? ?? Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator. 8.? ?? To establish a culture from the frozen state place the vial in a 35°C water bath.? Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.?? Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC? 700831) or Enterobacter aerogenes (ATCC? 13048). 9. Incubate at 25°C with the cap screwed on tightly. 10. Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 ml to 10.0 ml of bacterized ATCC medium 1525. Follow the protocol for maintenance of culture. |
| Name of Depositor | TA Nerad |
| References | Carr M, et al. Molecular phylogeny of choanoflagellates, the sister group to Metazoa. Proc. Natl. Acad. Sci. USA. 105: 16641-16646, 2008. PubMed: 18922774 Dayel MJ, et al. Cell differentiation and morphogenesis in the colony-forming choanoflagellate Salpingoeca rosetta. Dev. Biol. 357: 73-82, 2011. PubMed: 21699890 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
