| 產品名稱 |
RA-1.2 |
| 商品貨號 |
B209313 |
| Designations |
RA-1.2 |
| GenBank Number |
U31346
|
| Species |
Homo sapiens, human |
| Depositors |
H Menard |
| Applications |
This clone expresses a fusion protein of GST and a truncated calpastatin. |
| Vector |
Construct size (kb): 4.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAST
Gene name: calpastatin
Contains complete coding sequence?: N
Chromosome: 5; Localization: 5 q15-q21
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.323
Cross references: DNA Seq. Acc.: U31346
Nucleotides 1-1323 of the insert correspond to
nucleotides 1-1323 of U31346. Insert lengths(kb): 1.322999954223633 Tissue: placenta Gene product: calpastatin [CAST] |
| Insert Size (kb) |
1.323 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.3; BamHI--4.0; HindIII--3.0, 0.65, 0.35; PstI--3.1, 0.9; EcoRI/PstI--2.7, 0.9, 0.5. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.30,0.87,1.15; PstI 0.42. This clone expresses a fusion protein of GST and a truncated calpastatin. This calpastatin begins at nt1677 of D16217 and continues to the poly(A) tail, but the 3' UTR is different. |
| References |
Despres N, et al. Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera. J. Clin. Invest. 95: 1891-1896, 1995. PubMed: 7706496
|
