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The 764 bp PstI fragment (derived from the open reading frame) has been used as a probe for the human and rat sequences.

Construct size (kb): 4.639999866485596

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GCS*
Gene name: gamma-glutamylcysteine synthetase
Contains complete coding sequence?: U
Tissue: kidney
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.679
Cross references: DNA Seq. Acc.: M90656

Insert lengths(kb): 1.679000020027161

Tissue: kidney

Gene product: gamma-glutamylcysteine synthetase( glutamylcysteine synthetase, gamma, gamma-glutamylcysteine synthetase, light subunit) [GCS*]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.65; BglII--4.65; HindIII--4.65; PvuII--2.45, 1.45, 0.75; PstI--3.7, 0.8, 0.15.

The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.463; NcoI--0.65; PstI--0.14, 0.9; PvuII--0.44; SacI--0.9; XbaI--1.44.

The first 120 bp of the insert do not match the corresponding sequences of the full-length cDNA and presumably represent cloning artifacts.

Detects human transcripts of 4.0 and 3.2 kb.

The 764 bp PstI fragment (derived from the open reading frame) has been used as a probe for the human and rat sequences.

Encodes 387 aa (of 637 aa) at the carboxyl end of the protein, and includes 398 nt of 3' untranslated sequence. The portion of this clone derived from the cDNA therefore corresponds approximately to nt 845 - 2400 of the sequence record.