| Vector |
Construct size (kb): 44.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaFIX II
Intact vector size: 41.900
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: NotI SalI XhoI EcoRI
Polylinker sites: XbaI SacI NotI SacI SalI XhoI EcoRI XbaI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter: T3, T7
replicon: lambda
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SSTR2
Gene name: somatostatin receptor 2
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q24
Tissue: placenta, male Caucasian
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 15.0
Cross references: DNA Seq. Acc.: M81830 Insert lengths(kb): 15.0 Tissue: placenta, male Caucasian Gene product: somatostatin receptor 2 [SSTR2] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--26.5, 23.0, 2.0; XhoI-->23.0, 14.0; SacI--25.0, 16.5, 3.3, 2.9, 1.4, 1.2, 0.8; SalI--25.0, 15.5, 6.3, 4.4; XbaI--20.0, 9.2, 7.0, 3.9, 3.7, 2.2, 2.0, 1.8, 1.6. Detects a murine genomic EcoRI fragment of 8.2 kb. Additional GenBank/EMBL accession number: L05521. Detects human transcripts of 8.5 and 2.5 kb. |
| References |
Yamada Y, et al. Cloning and functional characterization of a family of human and mouse somatostatin receptors expressed in brain, gastrointestinal tract, and kidney. Proc. Natl. Acad. Sci. USA 89: 251-255, 1992. PubMed: 1346068
Yamada Y, et al. Human somatostatin receptor genes: localization to human chromosomes 14, 17, and 22 and identification of simple tandem repeat polymorphisms. Genomics 15: 449-452, 1993. PubMed: 8449518
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