Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
contaminant in CCAP 950/1 culture of Rhizochromulina marina, 1987
Product Format
frozen
Type Strain
no
Comments
Ultrastructure of flagellar apparatus
Medium
ATCC® Medium 1525: Seawater 802 medium
Growth Conditions
Temperature: 25.0°C
Protocol: ATCCNO: 50561 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 10 ml of bacterized ATCC medium 1525 in a T-25 tissue culture flask. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly and incubate horizontally at 25C. The culture should be ready to subculture in 5-7 days. To subculture, vigorously agitate the flask and aseptically transfer a 0.1 ml aliquot to 10 ml of bacterized ATCC medium 1525 containing a single rice grain and incubate as above. ATCC medium 1525 is bacterized by inoculating the medium with a loopful of Klebsiella pneumoniae subsp. pneumoniae ATCC 27889 (a non-pathogenic strain) from an ATCC medium 3 slant approximately 24 hours before using. Other strains of Klebsiella or other species of bacteria may serve equally well as a food source. This must be determined empirically.
Subcultivation
Protocol: ATCCNO: 50561 SPEC: This strain is routinely shipped as a frozen stabilate. Thaw the ampule and aseptically transfer the material to 10 ml of bacterized ATCC medium 1525 in a T-25 tissue culture flask. DO NOT distribute the thawed material to a larger volume of medium. It is essential to first establish the culture in a small volume. Screw cap on tightly and incubate horizontally at 25C. The culture should be ready to subculture in 5-7 days. To subculture, vigorously agitate the flask and aseptically transfer a 0.1 ml aliquot to 10 ml of bacterized ATCC medium 1525 containing a single rice grain and incubate as above. ATCC medium 1525 is bacterized by inoculating the medium with a loopful of Klebsiella pneumoniae subsp. pneumoniae ATCC 27889 (a non-pathogenic strain) from an ATCC medium 3 slant approximately 24 hours before using. Other strains of Klebsiella or other species of bacteria may serve equally well as a food source. This must be determined empirically.
Name of Depositor
CJ O'Kelly
Year of Origin
1987
References
O'Kelly CJ, Patterson DJ. The flagellar apparatus of Cafeteria roenbergensis Fenchel & Patterson, 1988 (Bicosoecales = Bicosoecida). Eur. J. Protistol. 32: 216-226, 1996.
