A 2.0 kb BamHI fragment of the insert has been used to detect a mRNA of about 4.3 kb which was present at higher concentration in galactose-grown Saccharomyces.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following sites separated by (kb)(approx): SstI- 0.5- HindIII- 0.4- BamHI- 0.9- HindIII- 1.2- BamHI- 1.3- EcoRI- 0.5- EcoRI.
Carries plasmid pG70/T1 with wild-type gene
A regulatory element has been demonstrated between -354 and -143 (relative to +1 being the A of the ATG initiation signal).
A 2.0 kb BamHI fragment of the insert has been used to detect a mRNA of about 4.3 kb which was present at higher concentration in galactose-grown Saccharomyces.
pG70/T1 is available in Escherichia coli RR1 (ATCC 37661) and in Saccharomyces cerevisiae C225/U2 (ATCC 66320).
Repetto B, Tzagoloff A. Structure and regulation of KGD1, the structural gene for yeast alpha-ketoglutarate dehydrogenase. Mol. Cell. Biol. 9: 2695-2705, 1989. PubMed: 2503710
