Insert contains the following sites separated by (kb): EcoRI- 0.15- PstI- 0.7- SstI- 0.35- SstI- 0.08- SmaI- 0.58- PstI- 0.24- HindIII- 0.18- SstI- 0.18- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.05, 2.7; PstI--3.2, 1.9, 0.73; HindIII--5.3, 0.49; SmaI--4.4, 1.45; SacI--4.25, 1.3. The insert contains the complete coding sequence plus 5' and 3' untranslated regions. |
