| 產(chǎn)品名稱(chēng) |
Rubella virus |
| 商品貨號(hào) |
B206835 |
| Classification |
Togaviridae, Rubivirus |
| Agent |
Rubella virus |
| Strain |
M33 |
| Common Name |
Rubella virus |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Throat washings from Army recruit, New Jersey, 1961 |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Rubella virus is 40-80 nm in diameter and ether sensitive.
Vials contain total fluid and material from cell culture infected by virus. The vials will contain cellular debris and possibly cells containing cell-associated virus. |
| Effect on Host |
No CPE produced on production host. Recognized primarily by interference for other viruses (GMK), or production of CF and HA antigens. |
| Recommended Host |
BS-C-1 (ATCC® CCL-26™) |
| Growth Conditions |
Temperature: 35°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 60-70% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 11-15 days |
| Name of Depositor |
PD Parkman, Walter Reed Army Institute of Research |
| Source |
Throat washings from Army recruit, New Jersey, 1961 |
| Year of Origin |
1961 |
| References |
Parkman PD, et al. Recovery of rubella virus from army recruits. Proc. Soc. Exp. Biol. Med. 111: 225-230, 1962. PubMed: 13941530
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