| 產(chǎn)品名稱 |
HFBEI03 |
| 商品貨號 |
B206670 |
| Designations |
HFBEI03 |
| GenBank Number |
X72727
T07375
|
| Species |
Homo sapiens, human |
| Vector |
Construct size (kb): 5.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HNRPK
Gene name: heterogenous nuclear ribonucleoprotein K
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.3
Cross references: DNA Seq. Acc.: T07375; EST: EST05264; Primer: M13-21; THC
id: THC122448
DNA Seq. Acc.: X72727; EST: EST05264
Nucleotides 1-311, 287-360 of the insert correspond to
nucleotides 2277-1967, 1992-1929 of X72727 with 95% identity. Insert lengths(kb): 2.299999952316284 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: heterogeneous nuclear ribonucleoprotein complex K [HNRPK] |
| Insert Size (kb) |
2.300 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.3. Based on the alignment with GenBank X72727 (2830 nt, CDS 210-1604) and the insert size, this clone appears to contain most if not all of the coding sequence. |
| References |
Adams MD, et al. 3400 expressed sequence tags identify diversity of transcripts from human brain. Nat. Genet. 4: 256-267, 1993. PubMed: 8358434
|
