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Construct size (kb): 3.799999952316284

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PLA2G2A
Other gene symbols: PLA2L; PLA2B
Gene name: phospholipase A2, group IIA (platelets, synovial fluid)
Contains complete coding sequence?: U
Chromosome: 1; Localization: 1
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.8
Cross references: DNA Seq. Acc.: M86400; EST: EST03131
Nucleotides 1-193 of the insert correspond to
nucleotides 2581-2388 of M86400 with 100% identity.
DNA Seq. Acc.: T05242; EST: EST03131; Primer: M13-21

Insert lengths(kb): 0.800000011920929

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: phospholipase A2, group IIA (platelets, synovial fluid)(phospholipase A2 polypeptide B (synovial fluid)) [PLA2B]

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8.

Based on the insert size and the match to the published sequence, probably a partial clone consisting primarily of 3' untranslated region, because the coding sequence of M86400 is nt 85-822.