| Comments |
Restriction digests of the clone give the following sizes (kb): AccI--5.4; EcoRI--5.4; BamHI--3.2, 2.3; HindIII--4.3, 1.15; EcoRI/HindIII--2.7, 1.45, 1.15. This probe is the same as pTHH39. Codominant segregation of RsaI RFLP demonstrated in more than 60 families. RFLPs observed under normal stringency (0.1X SSC). Enzyme(s) not detecting polymorphism: MspI, TaqI, BglII, PstI, PvuII, EcoRI, BamHI, HindIII. |
