Restriction digests of the clone give the following sizes (kb): BamHI--7.6; PstI--7.6; BamHI/XhoI--7.0, 0.5. Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker. |
