Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; PstI--3.6, 1.4; BamHI--5.0; HindIII--3.8, 0.9, 0.17, 0.13; EcoRI/BamHI--3.0, 2.0. The insert contains the following restriction sites (nt from the 5' end): PstI--756; HindIII--1056, 1194, 2014; XbaI--1360. The insert extends from nt 509-2568 of the reported sequence, including exons 4-13. Transcription from the T7 promoter gives the sense strand. |
