| 產(chǎn)品名稱(chēng) |
c9581 |
| 商品貨號(hào) |
B203722 |
| Designations |
c9581 |
| GenBank Number |
U18778
U18796
|
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
R Hyman |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pHC79
Intact vector size: 6.430
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI BamHI SalI EcaI PstI
Polylinker sites:
Construction: pBR322, Charon 4A
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, tetR
replicon: pMB1
terminator: none
Cross references: DNA Seq. Acc.: L08873 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae
Gene symbol: GPA2
Gene name: nucleotide binding regulatory protein
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: SRB4
Gene name: RNA polymerase II suppressor
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: PRO3
Gene name: delta 1-pyrroline-5-carboxylate reductase
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: GCD11
Gene name: gamma subunit of translational initiation factor eIF-2
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: CHO1
Gene name: phosphatidylserine synthase
Contains complete coding sequence?: Y
Chromosome: V; Localization: V R, 23 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: GAL83
Gene name: glucose repression protein
Contains complete coding sequence?: Y
Chromosome: V; Localization: V R, 11 cM from CEN
Genome: Saccharomyces cerevisiae
Gene symbol: YPT8
Gene name: Rab-family GTPase activating protein
Contains complete coding sequence?: Y
Chromosome: V
Genome: Saccharomyces cerevisiae
Gene symbol: GLN3
Gene name: positive nitrogen regulatory protein
Contains complete coding sequence?: Y
Chromosome: V
Strain: AB972 (ATCC 76269)
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb):
Cross references: DNA Seq. Acc.: U18778
Nucleotides ? of the insert correspond to
nucleotides 37895-66030 of U18778.
DNA Seq. Acc.: U18796
Nucleotides ? of the insert correspond to
nucleotides 1-14353 of U18796. Gene product: phosphatidylserine synthase [CHO1] |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: frozen, uncharacterized |
| Comments |
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
