Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age
adult
Gender
female
Applications
The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon.
The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon.
Storage Conditions
liquid nitrogen vapor phase
Derivation
-
Clinical Data
female
Comments
The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon. The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)
Subculturing
Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks.
Interval: Subculture every 10 to 17 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium Renewal: Once per week.
Cryopreservation
Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 100%
Temperature: 20.0°C
Name of Depositor
J Rolland, J Winton, B Krossoey
Deposited As
Salmo salar
Year of Origin
1998
References
Devold M, et al. Use of RT-PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. Dis. Aquat. Org. 40: 9-18, 2000. PubMed: 10785858
