| Vector |
Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: amyloid protein homolog
Contains complete coding sequence?: N
Chromosome: 11; Localization: 11, 610.580000 cR
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: L23114; EST: EST06905
Nucleotides 1-316 of the insert correspond to
nucleotides 760-1075 of L23114 with 98% identity.
DNA Seq. Acc.: T09012; EST: EST06904; Primer: M13-21; THC
id: THC104345
DNA Seq. Acc.: T09013; EST: EST06905; Primer: M13 Reverse;
THC id: THC121039
DNA Seq. Acc.: Z22572; EST: EST06904
Nucleotides 360-1 of the insert correspond to
nucleotides 2355-2714 of Z22572 with 98% identity.
DNA Seq. Acc.: Z22572; EST: EST06905
Nucleotides 52-316 of the insert correspond to
nucleotides 1158-1422 of Z22572 with 98% identity. Insert lengths(kb): 1.799999952316284 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: amyloid protein homolog |
