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Construct size (kb): 5.550000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CLU
Other gene symbols: APOJ
Genomic copy number: unique
Gene name: clusterin (complement lysis inhibitor, SP-40,40, sulfated
glycoprotein 2, testosterone-repressed prostate message 2,
apolipoprotein J)
Contains complete coding sequence?: U
Chromosome: 8; Localization: 8 p21
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.6
Cross references: DNA Seq. Acc.: J02908
Nucleotides 1-287 of the insert correspond to
nucleotides 206-492 of J02908 with 98% identity.
DNA Seq. Acc.: M14723
Nucleotides 1-287 of the insert correspond to
nucleotides 335-621 of M14723 with 98% identity.
DNA Seq. Acc.: M25915
Nucleotides 1-287 of the insert correspond to
nucleotides 483-769 of M25915 with 98% identity.
DNA Seq. Acc.: M64722
Nucleotides 1-287 of the insert correspond to
nucleotides 240-626 of M64722 with 98% identity.
DNA Seq. Acc.: M74816
Nucleotides 1-287 of the insert correspond to
nucleotides 110-396 of M74816 with 98% identity.
DNA Seq. Acc.: M78166; EST: EST01759; Primer: M13 Forward;
THC id: THC103444

Insert lengths(kb): 2.599999904632568

Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted

Gene product: clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J) [CLU]

Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 2.0, 0.6.

EST01759 matches GenBank accession HUMAPOJ with 98.25% identity over the sequence range: 1-287.