Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0. The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014. A high copy number vector useful for direct cloning of double stranded amplification products. Digestion of the vector with XcmI produces a linear molecule with 3' unpaired deoxythymidine residues at both ends, suitable for direct cloning of PCR products. |
