| 產品名稱 |
pCT5-MK18 S26:A |
| 商品貨號 |
B202720 |
| Designations |
pCT5-MK18 S26:A |
| Species |
Mus musculus, mouse |
| Depositors |
S Paul |
| Applications |
produces protein immunoglobulin light chain, anti-VIP |
| Vector |
Construct size (kb): 5.300000190734863 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.800000011920929 Tissue: hyperimmunized spleen, B-lymphocytes Gene product: immunoglobulin light chain Target Gene: immunoglobulin light chain, anti-VIP |
| Insert Size (kb) |
0.800 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 2.1; BstEII--5.3; BamHI--5.3; SfiI/NotI--4.5, 0.8. Insert was isolated from hybridoma cell line clone c23.5 producing anti-VIP (vasoactive intestinal peptide) monoclonal antibodies. Insert encodes the following mutant form: Ser26 --> Ala. The following nucleotides differ from the published sequence, but do not alter the amino acid sequence: nt 3 T->C; nt 6 G->T; nt 9 G->A. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Gao QS, et al. Molecular cloning of a proteolytic antibody light chain. J. Biol. Chem. 269: 32389-32393, 1994. PubMed: 7798238
Gao QS, et al. Site-directed mutagenesis of proteolytic antibody light chain. J. Mol. Biol. 253: 658-664, 1995. PubMed: 7473741
|
