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Leishmania tropica (Wright) Luhe

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編號:B202634

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產品名稱	Leishmania tropica (Wright) Luhe
商品貨號	B202634
Deposited As	Leishmania adleri
Strain Designations	WHO Israel LRC 146
Application	Vector borne research
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	lizard, Latastia longicauda revoili, Kenya
Product Format	frozen
Type Strain	no
Comments	Taxonomy (ATCC lab.). Promastigotes. In vitro phagocytosis by lizard leukocytes Taxonomic status Variation in kinetoplast minicircle and maxicircle DNA sequences Cyclopropane fatty acid
Medium	ATCC^® Medium 811: Leishmania medium
Growth Conditions	Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Subcultivation	Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly.
Name of Depositor	J Janovy
Chain of Custody	ATCC <<--J Janovy<<--WHO LRC Jerusalem <<--- . . . <<--- Heisch?
References	Dollahon NR, Janovy J Jr.. Leishmania adleri: in vitro phagocytosis by lizard leukocytes and peritoneal exudate cells. Exp. Parasitol. 34: 56-61, 1973. PubMed: 4722485 Dollahon NR, Janovy J Jr.. Experimental infection of New World lizards with Old World lizard Leishmania species. Exp. Parasitol. 36: 253-260, 1974. PubMed: 4413377 Simpson L. The status of Leishmania tarentolae/Trypanosoma platydactyli. Parasitol. Today 4: 115-118, 1988. Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 Gomez-Eichelmann MC, et al. Comparison of several lizard Leishmania species and strains in terms of kinetoplast minicircle and maxicircle DNA sequences, nuclear chromosomes, and membrane lipids [published erratum appears in Mol. Biochem. Parasitol. 28: 301, 1988]. Mol. Biochem. Parasitol. 27: 143-158, 1988. PubMed: 3344003

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