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1. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the appropriate volume of Phenol Red to complete media. Dilute 1:1000 when using to supplement complete media; i.e., 0.5 mL of phenol red per 500 mL of complete cell-specific media.
2. Tightly cap the bottle (or other vessel) of supplemented growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the vessel.
3. Fully supplemented media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, fully supplemented media is stable for 30 days.

A Certificate of Analysis (COA) is available upon request for each lot of Phenol Red.