Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 100 mL
Storage Conditions
-20°C (or -70°C for long-term storage)
Comments
The Chondrocyte Differentiation Tool is a complete differentiation medium designed to induce chondrogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells with high efficiency.
The Chondrocyte Differentiation Tool provides enough medium to differentiate up to ~ 13.5 x 107 cells, sufficient to seed 20 wells of a 48-well tissue culture plate when using alginate encapsulation.
Chondrocyte differentiation requires that the cells be grown in a three-dimensional aggregate cell culture. Micromass culture can be used, but for the best results, ATCC recommends the use of a matrix, such as alginate, to provide a scaffold for the deposition of proteoglycans. Detailed instructions for the formation of chondrogenic microbeads are provided.
Complete Growth Medium
Antimicrobials and phenol red are not required but may be added to the Chondrocyte Differentiation Tool if desired prior to use. The recommended volume of each optional component to be added to Chondrocyte Differentiation Tool is summarized in Table 1.
Table 1. OptionalAddition of Antimicrobials/Antimycotics and Phenol Red per 100 mL of Medium
