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<em>Scheffersomyces shehatae</em> (Buckley et van Uden) Urbina et Blackwell

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編號:B200818

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產品名稱	Scheffersomyces shehatae (Buckley et van Uden) Urbina et Blackwell
商品貨號	B200818
Deposited As	Candida shehatae Buckley et van Uden, anamorph
Strain Designations	M 175 [NRC 2883]
Application	Bioethanol production
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	no
Preceptrol®	no
Genome Sequenced Strain	Yes
Comments	Ferments xylose Increase in ploidy during stress Genome sequencing strain (Nanjing Forestry University, China)
Medium	ATCC^® Medium 28: Emmons' modification of Sabouraud's agar ATCC^® Medium 200: YM agar or YM broth ATCC^® Medium 1245: YEPD
Growth Conditions	Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTTAACTGCGCGGCGAAAAAACCTTACACACAGTGTTTTCTTTATTAGAAACTATTGCTTTGGTCTGGCTTAGAAATAAGTTGGGCCAGAGGTTAACCAAACTTCAATTTTATTATTGAATTGTTATTTTATTTAATTTGTCAATTTGTTGATTAAATTCAAAAAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGATTTTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAGAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAGCCCTCGGGTTTGGTATTGAGTGATACTCTTAGTCAGACTAGGCGTTTGCTTGAAAAGTATCGGCATGAGTAGTACTAGATAGTGCTTTCAGGATATTTCAATGTATTAGGTTTATCCAACTCGTTGAGAATTCTTGGTAGTGAATTTTTAGTATCATGGCTCTGCCTTACAAAACAACAAACAAGTTTGACCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGTCTTAGTAACGGCGAGTGAAGCGACAAAAGCTCAAATTTGAAATCTGGCACCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGAGTTGGCTCTTGTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCCAACTCTATGTAAAGTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTTTGAGATCAGACTTGGTATTTTGTATGTCTTGCTTTCGGGTGGGGCCTCTGCAGTTTACTGGGCCAGCATCGGTTTGGACGGTAGGATAATGACATTGGAATGTGGCACTACTTCGGTGGTGTGTTATAGACTTTGTTGATACTGCCTGTCTAGACCGAGGACTGCGTCTTTGACTAGGATGCTGGCATAATGATCCTAAACCGC
Name of Depositor	SA Meyer
Cross References	Nucleotide (GenBank) : JQ023135 RPB 1 gene Nucleotide (GenBank) : HQ652024 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : HQ652065 ITS including 5.8S rRNA gene Nucleotide (GenBank) : JQ023147 RPB 2 gene Nucleotide (GenBank) : KC479677 18S rRNA gene Nucleotide (GenBank) : KC507458 EF-1 alpha gene Nucleotide (GenBank) : KC479711 XYL1 gene
References	Alexander MA, et al. Levels of pentose phosphate pathway enzymes from Candida shehatae grown in continuous culture. Appl. Microbiol. Biotechnol. 29: 282-288, 1988. Hinfray CT, et al. Influence of the oxygenation level on D-xylose fermentation by free and agar-entrapped cultures of C. shehatae. Appl Microbiol Biotechnol 42: 682-687, 1995 Jeffries TW. Effects of culture conditions on the fermentation of xylose to ethanol by Candida shehatae. Biotechnol. Bioeng. Symp. 15: 149-166, 1985. Lee H, Fisher BG. Unusual fructose utilization by Pichia stipitis and its potential application. J. Ferment. Bioeng. 69: 79-82, 1990. Palnitkar S, Lachke A. Effect of nitrogen sources on oxidoreductive enzymes and ethanol production during D-xylose fermentation by Candida shehatae. Can. J. Microbiol. 38: 258-260, 1992. PubMed: 1393828 Suh SO, et al. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov., and Candida manassasensis sp. nov., four novel yeasts associated with wood- ingesting insects and their ecological and biofuel implications. Int. J. Syst. Evol. Microbiol. 63: 4330-4339, 2013. PubMed: 24014624 Talbot NJ, Wayman M. Increase in ploidy in yeasts as a response to stressing media. Appl Microbiol Biotechnol 32: 167-169, 1989 Yang VW, Jeffries TW. Purification and properties of xylitol dehydrogenase from the xylose-fermenting yeast Candida shehatae. Appl Biochem Biotechnol 26: 197-206, 1990

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