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<em>Trichoderma afroharzianum</em> Chaverri et al.

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產(chǎn)品名稱	Trichoderma afroharzianum Chaverri et al.
商品貨號	B200722
Deposited As	Trichoderma harzianum Rifai
Strain Designations	1295-22 [T-22]
Application	Biological control of Fusarium graminearum Biological control of Rhizoctonia solani Biological control of Sclerotium rolfsii Biological control of Pythium ultimum Promotes plant growth and vigor and improves the efficiency of root systems
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	freeze-dried
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	no
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol®	no
Comments	The strain was recently re-identified as Trichoderma afroharzianum, a close sister of Trichoderma harzianum, based on the multigene sequence analysis.
Morphology	On PDA medium at 25°C after 8 days, mycelium white becoming green to dark green, tomentose. Reverse reddish brown. Hyphae hyaline, guttulate. Conidiophores with phialides in whorls of 3-4. Phialides 9-10.5 X 2.5-3 μm. Conidia green, globosa to subglobose, smooth, 3-6 X 2.5-5.5 μm. Chlamydospores terminal and intercalary.
Medium	ATCC^® Medium 336: Potato dextrose agar (PDA)
Growth Conditions	Temperature: 25°C Atmosphre: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGGATCTCTGCCCCGGGTGCGTCGCAGCCCCGGACCAAGGCGCCCGCCGGAGGACCAACCAAAACTCTTATTGTATACCCCCTCGCGGGTTTTTTTATAATCTGAGCCTTCTCGGCGCCTCTCGTAGGCGTTTCGAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGATCGGCCCTGCCTTGGCGGTGGCCGTCTCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACACTCGCATCGGGAGCGCGGCGCGTCCACAGCCGTTAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGTCCCTAGGGCCCGAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTCTGGCTGGCCGCCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACCTTGAAAAGAGGGTTAAATAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGTGACCAGACTTGGGCGCGGCGGATCATCCGGGGTTCTCTCCGGTGCACTTCGCCGCGTCTAGGCCAGCATCAGTTCGTCGCGGGGGAAAAAGGCTTCGGGAACGTGGCTCCTCCGGGAGTGTTATAGCCCGTTGCATAATACCCTGCGGTGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCACCAGCGAC elongation factor (EF) 1-alpha gene CACTGGTACTTCCCAGGCCGATTGCGCTATCCTCATCATTGCCGCCGGTACTGGTGAGTTCGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCACGCTCTGCTCGCCTACACCCTGGGTGTTAAGCAGCTCATCGTTGCCATCAACAAGATGGACACTGCCAACTGGGCCGAGGCTCGTTACCAGGAAATCATCAAGGAGACTTCCAACTTCATCAAGAAGGTCGGCTTCAACCCCAAGGCTGTTGCTTTCGTCCCCATCTCCGGTTTCAACGGTGACAACATGCTCCAGCCCTCCACCAACTGCCCCTGGTACAAGGGCTGGGAGAAGGAGACCAAGGCTGGCAAGTTCACCGGCAAGACCCTCCTTGAGGCCATCGACTCCATCGAGCCCCCCAAGCGTCCCACGGACAAGCCCCTCCGTCTTCCCCTCCAGGATGTCTACAAGATCGGTGGTATTGGAACAGTTCCCGTCGGCCGTATCGAGACTGGTGTCCTCAAGCCCGGTATGGTCGTCACTTTCGCTCCCTCCAACGTCACCACTGAAGTCAAGTCCGTCGAGATGCACCACGAGCAGCTCGTCGAGGGTGTTCCCGGTGACAACGTTGGTTTCAACGTCAAGAACGTTTCCGTTAAGGAAATTCGCCGTGGTAACGTTGCCGGTGACTCCAAGAACGACCCCCCCATGGGTGCCGCTTCTTTCACCGCTCAGGTCATCGTCATGAACCACCCTGGCCAGGTCGGTGCCGGCTACGCCCCCGTTCTTGACTGCCACACTGCCCACATTGCCTGCAAGTTCGCCGAGCTCCAGGAGAAGATCGACCGCCGTACCGGTAAGGCTACCGAGACTGCCCCCAAGTTCATCAAGTCCGGTGACTCTGCCATCGTCAAGATGATTCCCTCCAAGCCCATGTGCGTTGAGGCTTTCACCGACTACCCTCCCCTGGGTCGTTTCGCCGTCCGTG calmodulin gene TCTTTGTGAGTCTTTAACCTCGTTTCTGGTGGCAATTCGGACGCTTTGAGTGCTAACCCAGCTGTTACAGGACAAGGACGGTGATGGTACGTAGCAGCGAGTGACGCGACACCACCCCTCTCCCCCCTTTGCGAACAGGCACCGGAGCACCGAATGACTTGCTTCCGACCCATATCTTCCTTGACAACCCCTTTTCATGATATCTGAGCAAAGAAACGAACTGACAGGCTTGACCCCGCAGGCCAGATCACCACCAAGGAGCTGGGCACCGTGATGCGCTCCCTCGGCCAGAACCCCTCCGAGTCAGAGCTGCAGGACATGATTAACGAGGTCGATGCCGACAACAACGGATCTATCGATTTCCCCGGTATGTCAAAAGTCAAAGTGTTGGGTCGTTCCCATATTCGAGCTAATACGGAGCGGTGTAGAATTCCTGA
Name of Depositor	Cornell Res. Fndn., Inc.
U.S. Patent Number	5,260,213
Isolation	Protoplast fusion of ATCC^® 20737™ and ATCC^® 60850™
Cross References	Nucleotide (GenBank) : FJ545255 ITS including 5.8S rRNA gene Nucleotide (GenBank) : KU933424 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : KU933430 elongation factor (EF) 1-alpha gene Nucleotide (GenBank) : KU933406 calmodulin gene
References	Altomare C, et al. Solubilization of phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22. Appl. Environ. Microbiol. 65: 2926-2933, 1999. PubMed: 10388685 Lo CT, et al. Ecological studies of transformed Trichoderma harzianum strain 1295-22 in the rhizosphere and on the phylloplane of creeping bentgrass. Phytopathology 88: 129-136, 1998. Stasz TE, et al. Fused biocontrol agents. US Patent 5,260,213 dated Nov 9 1993

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