| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PPP3CB
Other gene symbols: CALNA2
Genomic copy number: unique
Gene name: protein phosphatase 3 (formerly 2B), catalytic subunit,
beta isoform (calcineurin A beta)
Contains complete coding sequence?: U
Chromosome: 10; Localization: 10; 10, 448.339999 cR
Tissue: brainstem
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: M29551 Insert lengths(kb): 1.799999952316284 Tissue: brainstem Gene product: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)(protein phosphatase 3, catalytic subunit, beta isoform) [CALNA2] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--24.2, 19.5, 1.8; ClaI--12.5, 6.0 (doublet), 4.0 (doublet), 3.3, 2.6, 1.85, 1.6, 1.1 (doublet), 0.95, 0.6, 0.35; HindIII-->23.0, 9.4, 7.2, 3.8, 0.56; PstI--12.5 (doublet), 4.7, 3.1, 2.85, 2.5, 2.0, 1.7, 1.15, 1.1, 0.8, 0.55, 0.48, 0.37 +smaller; PvuII-->23.0, 4.4, 4.2, 3.6, 2.7, 1.75, 1.2, 0.95, 0.6, 0.52. The insert contains the following restriction sites (approximate kb from the 5' end): ClaI--0.67; HindIII--0.37, 0.38; NcoI--0.11; PstI--1.01, 1.24; PvuII--1.00; SacI--0.68. Corresponds to nt 311-2180 of the GenBank record, and includes the 3' portion of the coding region (nt 117-1691). This was derived from the more prevalent type II isoform, and lacks 54 bp and encodes a different carboxy terminus relative to the type I. |
