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Trichoderma atroviride Karsten sensu Bissett

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編號:B200298

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產品名稱	Trichoderma atroviride Karsten sensu Bissett
商品貨號	B200298
Deposited As	Trichoderma harzianum Rifai
Strain Designations	107 P1 [P1]
Alternate State	Hypocrea atroviridis Dodd et al.
Application	Produces chitin 1,4-beta-chitobiosidase chitobiosidase Produces endo-1,3(4)-beta-glucanase endo-1,3-beta-glucanase, laminarinase Produces endochitinase Produces glucan 1,3-beta-glucosidase beta-D-1,3-glucanase, exo-1,3-beta-glucanase, exo-1,3-beta-glucosidase Produces hexosaminidase A (alpha polypeptide) Hexose aminidase A (alpha polypeptide), beta-N-acetylhexosaminidase, N-acetyl-beta-glucosaminidase Biological control of Pythium spp. Biological control of Botrytis cinerea Produces a hydrophin-like protein in aerial spores
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format	frozen
Storage Conditions	Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Type Strain	no
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol®	no
Comments	Conidia that have already germinated at the time of application are better able to inhibit Botrytis cinerea than those that have not germinated, especially at low temperatures. chitinase gene expression ech42 gene expression before mycoparasitic contact
Medium	ATCC^® Medium 323: Malt agar medium ATCC^® Medium 325: Malt extract agar (Blakeslee's formula) ATCC^® Medium 336: Potato dextrose agar (PDA)
Growth Conditions	Temperature: 24°C to 26°C Atmosphere: Typical aerobic
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AGGGATCATTACCGAGTTTACAACTCCCAAACCCAATGTGAACCATACCAAACTGTTGCCTCGGCGGGGTCACGCCCCGGGTGCGTCGCAGCCCCGGAACCAGGCGCCCGCCGGAGGGACCAACCAAACTCTTTTCTGTAGTCCCCTCGCGGACGTTATTTCTTACAGCTCTGAGCAAAAATTCAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGGTCGGCGTTGGGGACCTCGGGAGCCCCTAAGACGGGATCCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCGCGTCCACGTCCGTAAAACACCCAACTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCTAGGGTCCGAGTTGTAATTTGTAGAGGATGCTTTTGGTGAGGTGCCGCCCGAGTTCCCTGGAACGGGACGCCGCAGAGGGTGAGAGCCCCGTCTGGCTGGCCACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTATATGTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACCTTGAAAAGAGGGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGTGACCAGACTTGGGCGCGGCGGATCATCCGGGGTTCTCCCCGGTGCACTTCGCCGTGTTCAGGCCAGCATCAGTTCGGCGCGGGGGAAAAAGGCTTCGGGAACGTGGCTCCTCCGGGAGTGTTATAGCCCGTTGCATAATACCCTGCGCTGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCACCAGCGA
Name of Depositor	Cornell Res. Fndn., Inc.
U.S. Patent Number	5,173,419
Special Collection	NCRR Contract
Isolation	Wood chips
Cross References	Nucleotide (GenBank) : U24158 cDNA clone Th1433 Nucleotide (GenBank) : L14614 Trichoderma harzianum endochitinase mRNA, complete cds. Nucleotide (GenBank) : S83231 nag1=N-acetyl-beta-D-glucosaminidase, genomic DNA, 3060 nt Nucleotide (GenBank) : AF049074 Trichoderma harzianum internal transcribed spacer 1, partial sequence
References	Lorito M, et al. Synergistic interaction between fungal cell wall degrading enzymes and different antifungal compounds enhances inhibition of spore germination. Microbiology 140: 623-629, 1994. PubMed: 8012584 Harman GE, et al. Antifungal synergistic combination of enzyme fungicide and non-enzymatic fungicide and use thereof. US Patent 5,433,947 dated Jul 18 1995 Mu?oz GA, et al. Comparison of aerial and submerged spore properties for Trichoderma harzianum. FEMS Microbiol Lett 125: 63-70, 1995 Lorito M, et al. Chitinolytic enzymes produed by Trichoderma harzianum: antifungal activity of purified endochitinase and chitobiosidase. Phytopathology 83: 302-307, 1993. Lorito M, et al. Purification, characterization, and synergistic activity of a glucan 1,3-beta-glucosidase and an N-acetyl-beta-glucosaminidase from Trichoderma harzianum. Phytopathology 84: 398-405, 1994. Klemsdal SS, et al. Isolation and characterization of a cDNA from Trichoderma harzianum P1 encoding a 14-3-3 protein homolog. Gene 171: 123-127, 1996. PubMed: 8675020 Zeilinger S, et al. Chitinase gene expression during mycoparasitic interaction of Trichoderma harzianum with its host. Fungal Genet. Biol. 26: 131-140, 1999. PubMed: 10328983 Mach RL, et al. Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals. Appl. Environ. Microbiol. 65: 1858-1863, 1999. PubMed: 10223970 Kuling C, et al. Enzyme diffusion from Trichoderma atroviride (= T. harzianum P1) to Rhizoctonia solani is a prerequisite for triggering of Trichoderma ech42 gene expression before mycoparasitic contact. Appl. Environ. Microbiol. 66: 2232-2234, 2000. PubMed: 10788407 Thrane C, et al. Substrate colonization, strain competition, enzyme production in vitro, and biocontrol of Pythium ultimum by Trichoderma spp. isolates P1 and T3. Eur. J. Plant Pathol. 106: 215-225, 2000. Hjeljord LG, et al. Antagonism of nutrient-activated conidia of Trichoderma harzianum (atroviride) P1 against Botrytis cinerea. Phytopathology 91: 1172-1180, 2001.

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