| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--23.0, 12.0, 6.6, 5.0, 3.7, 3.5; BamHI--23.0, 8.0 (doublet), 6.0, 4.2, 1.8; HindIII--23.0, 12.0, 8.0, 6.0 (doublet); BglII--23.0, 13.0, 8.2, 5.0, 2.1, 1.3; PvuI-->23.0, 16.0, 8.0, 5.0, 2.7. The HindIII and HincII polymorphisms have a combined PIC value of 0.67 and a combined heterozygosity of 71%. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, MspI, RsaI, BglII, PstI, MboI, AluI, BstI, EcoRV, KpnI, SacII, XmnI, PvuII (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Tsui LC, et al. Cystic fibrosis locus defined by a genetically linked polymorphic DNA marker. Science 230: 1054-1057, 1985. PubMed: 2997931
Knowlton RG, et al. A polymorphic DNA marker linked to cystic fibrosis is located on chromosome 7. Nature 318: 380-382, 1985. PubMed: 2999611
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Barker D, et al. Genetic linkage map of human chromosome 7 with 63 DNA markers. Proc. Natl. Acad. Sci. USA 84: 8006-8010, 1987. PubMed: 2891136
Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474
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