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Size (kb): 5.4520001411438000

DESCRIPTION OF VECTOR:
Intact vector size: 5.452
Type of vector: phagemid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho
Construction: pRS413, CUP1 promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): HIS3, ->
replicon: f1, <-
terminator: CYC1, <-
MCS: SpeI...XhoI, ->
promoter for expression: CUP1, ->
replicon: pMB1
marker(s): ampR, <-
centromere: CEN6
replicon: ARSH4

Vector: pCu413CUP1 (phagemid)

Promoters: Promoter for expression CUP1

Construction: pRS413, CUP1 promoter

Marker(s):ampR,HIS3

Construct size (kb): 5.4520001411438

Features: marker(s): HIS3
marker(s): ampR
promoter for expression: CUP1
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1
centromere: CEN6

cloning vector

Restriction digests of the clone give the following sizes (kb): SacI--5.4, SacI/SpeI--5.2, 0.26.

Gene CUP1 is the most potent for copper induction. CUP1 promoter can be used to create a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.

component of:ATCC 87778