Paramyxoviridae, Rubulavirus, Human parainfluenza virus 4
Deposited As
Parainfluenza 4a
Agent
Human parainfluenza virus 4a
Strain
M-25
Applications
Respiratory research
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Adapted to NCI-H292 cells from VR-279™.
Effect on Host
CPE, cell degeneration and rounding
Recommended Host
NCI-H292 (ATCC® CRL-1848™)
Growth Conditions
Temperature: 36°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-7 days
Name of Depositor
JC Hierholzer, Centers for Disease Control and Prevention
Source
Throat swab of patient with mild upper respiratory tract illness, 1958
References
Castells E, et al. NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses. Arch. Virol. 115: 277-288, 1990. PubMed: 2260924
Johnson KM, et al. Studies of a new human hemadsorption virus. I. Isolation, properties and characterization. Am. J. Hyg. 71: 81-92, 1960. PubMed: 14407482
