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Murine leukemia virus

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編號:B199433

品牌:Mingzhoubio

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產(chǎn)品名稱	Murine leukemia virus
商品貨號	B199433
Classification	Retroviridae, Gammaretrovirus, Mammalian virus group
Deposited As	Murine erythroleukemia virus
Agent	Murine leukemia virus
Strain	Rauscher
Applications	The R-SFFV clone can be rescued by superinfecting the host cells with Rauscher MuLV. Several weeks PI the cells will express R-SFFV and R-MuLV which can be injected into susceptible mice pretreated with phenyl hydrazin.
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	Biologically cloned NIH/3T3s infected with biologically cloned R-SFFV from Rauscher erythroleukemia cell line
Product Format	frozen
Storage Conditions	-70°C or colder
Comments	The R-SFFV clone can be rescued by superinfecting the host cells with Rauscher MuLV. Several weeks PI the cells will express R-SFFV and R-MuLV which can be injected into susceptible mice pretreated with phenyl hydrazin.
Effect on Host	No, in vitro effects: cytopathic effects in NIH/3T3 cells Yes, in vivo effects: rescued R-SFFV causes proliferation of hematopoietic stem cells of mice in vivo or in vitro
Recommended Host	Mus musculus mouse
Growth Conditions	Temperature: 37°C Incubation: 3 days
Name of Depositor	D Kabat
Special Collection	ATCC
Source	Biologically cloned NIH/3T3s infected with biologically cloned R-SFFV from Rauscher erythroleukemia cell line
References	Machida CA, et al. Reduced leukemogenicity caused by mutations in the membrane glycoprotein gene of Rauscher spleen focus-forming virus. J. Virol. 49: 394-402, 1984. PubMed: 6319740 Ruta M, Kabat D. Plasma membrane glycoproteins encoded by cloned Rauscher and Friend spleen focus-forming viruses. J. Virol. 35: 844-853, 1980. PubMed: 7420541 Ruta M, et al. A murine leukemia virus mutant with a temperature-sensitive defect in membrane glycoprotein synthesis. Cell 16: 77-88, 1979. PubMed: 421271 Stephenson JR, Aaronson SA. Characterization of temperature-sensitive mutants of murine leukemia virus. Virology 54: 53-59, 1973. PubMed: 4123462

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