vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--2.7; AvaI--2.7; BglI--1.6, 1.1.
Useful for rescuing cloned bacterial inserts by digestion with I-SceI, an enzyme recognizing a rare 18 bp sequence which has yet to be found in bacterial DNA.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Schweizer HP, Hoang TT. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene 158: 15-22, 1995. PubMed: 7789804
