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K20PR [TRGV9]

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編號:B198796

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產品名稱	K20PR [TRGV9]
商品貨號	B198796
Designations	K20PR [TRGV9]
GenBank Number	X03436
Species	Homo sapiens, human
Depositors	M Lefranc, TH Rabbitts
Vector	Construct size (kb): 3.599999904632568
Insert	DNA: genomic Insert lengths(kb): 1.029999971389771 Gene product: T cell receptor, gamma, variable region V9 [TCRGV9] Alleles: A2, A1
Insert Size (kb)	1.030
Media	ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information	Distributed: freeze-dried
Comments	Restriction digests of the clone give the following sizes (kb): EcoRI--3.6; HindIII--3.6; PstI--3.6; EcoRI/HindIII--2.5, 1.0; PstI/HindIII--3.6; PstI/EcoRI--2.7, 1.0. The V9 sequence (without J) is contained in a 410 bp PstI/AccI fragment. A 1.03 kb PstI/EcoRI fragment isolated from lambdaK20 and containing TCRGV9 gene rearranged to J1, cloned into a pUC vector (the number of the vector is not known).
References	Lefranc MP, et al. Rearrangement of two distinct T-cell gamma-chain variable-region genes in human DNA. Nature 319: 420-422, 1986. PubMed: 3003579 Huck S, Lefranc MP. Rearrangements to the JP1, JP and JP2 segments in the human T-cell rearranging gamma gene (TRGgamma)locus. FEBS Lett. 224: 291-296, 1987. PubMed: 2961609 Forster A, et al. New subgroups in the human T cell rearranging Vgamma gene locus. EMBO J. 6: 1945-1950, 1987. PubMed: 2820713 Marie-Paule Lefranc, personal communication

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