Restriction digests of the clone give the following sizes (kb): EcoRI--13.5; BglII--8.4, 3.1, 2.3; HindIII--13.5. Vector contains the following restriction sites (approximate kb from the HindIII site): BamHI--1.6; EcoRI--11.6; SstI--0.9, 11.9. Binary vector with plant selectable marker near the T-DNA left border and a glucuronidase (uidA) reporter gene. The marker and uidA are divergently transcribed, and can be easily removed or replaced. The order of the major features of this plasmid is: EcoRI - pAnos - SstI - uidA - SmaI - XbaI - SalI - HindIII - Pnos - BglII - dhfr - BamHI - pAg7 - T-DNA - BglII - RK2 ori - kanR. |
