| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; NdeI--5.4; BamHI/NdeI--4.6, 0.75; SphI--4.7, 0.65; PvuI--1.55, 1.35, 1.1, 1.1, 0.2. The insert contains the following restriction sites (approximate kb from the 5' end): PvuI--0.2, 0.45; SphI--0.35; PstI--0.39; EcoRV--0.68. Constructed by amplification from genomic DNA using the following primers that introduce, respectively, NdeI and BamHI sites: sense 5'-GCGCCCATATGTCCGCCAATC-3' and antisense 5'-GCTATAATTAGTTTGGATCCATCG-3'. |
| References |
Kohl L, et al. Triose-phosphate isomerase of Leishmania mexicana mexicana. Cloning and characterization of the gene, overexpression in Escherichia coli and analysis of the protein. Eur. J. Biochem. 220: 331-338, 1994. PubMed: 8125090
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