| 產品名稱 |
TFTA1 [3721.95.161.4] |
| 商品貨號 |
B197988 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the A chain (A1 and A2) of the plant toxin ricin |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the A chain (A1 and A2) of the plant toxin ricin |
| Comments |
Animals were immunized with ricin A chain. Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14). The antibody does not cross-react with the ricin B chain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
R Fulton |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Fulton RJ, et al. Purification of ricin A1, A2, and B chains and characterization of their toxicity. J. Biol. Chem. 261: 5314-5319, 1986. PubMed: 3957927
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