Restriction digests of the clone give the following sizes (kb): PstI/XbaI--3.3, 2.3, 1.9; BamHI--6.8, 0.9; EcoRI--5.4, 2.3; HindIII--5.8, 1.35, 0.6; XhoI--7.8. When cotransfected into CHO cells with pSV2neo, the construct expresses the receptor. The construct includes an XhoI/PstI fragment derived from a pBluescript SK+ multiple cloning sequence. The PstI site is at the 5' end of the cDNA. The PstI/XbaI fragment includes 350 bp of 5' untranslated and 220 bp of 3' untranslated sequence. |
