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Piscirickettsia salmonis Fryer et al.

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編號:B196859

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產品名稱	Piscirickettsia salmonis Fryer et al.
商品貨號	B196859
Classification	Thiotrichales, Piscirickettsiaceae, Piscirickettsia
Agent	Piscirickettsia salmonis Fryer et al.
Strain	LF-89
Biosafety Level	2 This is an exotic fish pathogen and caution should be exercised when working with this agent to avoid introducing it into environments where it has not been previously reported to exist. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	clinical specimen - animal Chile Isolation date: October, 1989
Product Format	frozen
Storage Conditions	-70°C or colder
Comments	This organism is a gram-negative, non-motile, pleomorphic 0.5 to 1.5 micometer diameter coccoid.
Effect on Host	Cytopathic effects in tissue culture (small refractile rounding and clumping) CPE; small refractile rounding and clumping
Growth Medium	Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate and 10 mM HEPES, 90%; fetal bovine serum, 10%
Recommended Host	Recommended Host:? CHSE-214 (ATCC^? CRL-1681^TM)
Growth Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 15°C to 18°C Duration: 6 to 14 days; For best results host cells should be 24 to 48 hours old and 70% - 80% confluent (not 100% confluent).
Name of Depositor	JL Fryer, CN Lannan
Special Collection	ATCC
Source	clinical specimen - animal Chile Isolation date: October, 1989
Geographical Isolation	South America
Year of Origin	October, 1989
References	Fryer JL, Mauel MJ. The rickettsia: an emerging group of pathogens in fish. Emerg. Infect. Dis. 3: 137-144, 1997. PubMed: 9204294 Fryer JL, et al. Isolation of a rickettsiales-like organism from diseased Coho salmon (Oncorhynchus kisutch) in Chile. Fish Pathol. 25: 107-114, 1990. VR-1361 is an exotic fish pathogen and caution should be exercised when working with this agent to avoid introducing it into environments where it has not been previously reported to exist. Fryer JL, et al. Piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes. Int. J. Syst. Bacteriol. 42: 120-126, 1992. PubMed: 1371057 Isolated by J. L. Fryer and C. N. Lannan from the kidney of a Coho salmon (Oncorhynchus kisutch) displaying lethargy, anemia, swollen kidney, splenomegaly, darkened coloration and rare hepatic lesions. small refractile rounding and clumping

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