| 產品名稱 |
pZP3.2 |
| 商品貨號 |
B196673 |
| Designations |
pZP3.2 |
| GenBank Number |
M20026
|
| Species |
Mus musculus, mouse |
| Depositors |
J Dean |
| Applications |
Analysis of the sequence record predicts the following restriction sites (nt from the 5' end): SmaI--186, 223; PstI--609, 950; PvuII--612; BalI--634, 1165; BglI--1167. |
| Vector |
Construct size (kb): 5.0 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.230000019073486 Tissue: oocyte Gene product: zona pellucida glycoprotein 3( zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C) [Zp3] Alleles: s, b |
| Insert Size (kb) |
1.230 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 1.3; PstI--3.4, 1.2, 0.4; BamHI--5.0; KpnI--5.0; BglI--2.2, 1.8, 1.0. Analysis of the sequence record predicts the following restriction sites (nt from the 5' end): SmaI--186, 223; PstI--609, 950; PvuII--612; BalI--634, 1165; BglI--1167. A nearly full-length cDNA, extending from nt 47-1275 (the coding region of the precursor extends from 30-1301 and the coding region of the predicted mature peptide extends from 96-1301). |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Lunsford RD, et al. Genomic mapping of murine Zp-2 and Zp-3, two oocyte-specific loci encoding zona pellucida proteins. Genomics 6: 184-187, 1990. PubMed: 1968044
Ringuette MJ, et al. Molecular analysis of cDNA coding for ZP3, a sperm binding protein of the mouse zona pellucida. Dev. Biol. 127: 287-295, 1988. PubMed: 3378665
|
