Restriction digests of the clone give the following sizes (kb): BglII--7.0; NsiI--2.2, 1.9, 1.2, 0.94; SphI--5.6, 1.4; XbaI--7.0. The insert contains the following restriction sites (approximate kb from the 5' end): PvuII--0.43, 0.46, 0.50, 0.58, 0.62; SphI--1.23. ATCC 69764 was deposited 3/17/95 to supplement ATCC 67077. Requests for ATCC 67077 should be referred to ATCC 69764. Constructed by excising the insert of pRG5 (ATCC 67076) with an EcoRI/HindIII digest, and blunt-ending the vector and insert with the Klenow fragment of DNA polymerase before ligation. Contains the complete coding sequence (nt 245-1414 of the sequence record). |
