| Vector |
Construct size (kb): 36.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SuperCos 1
Intact vector size: 7.600
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites: EcoRI NotI BamHI NotI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, neoR, G418R
promoter: T3, T7
replicon: pMB1, SV40
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D17S122
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 17; Localization: 17 p12-p11.2
Type of DNA: genomic
Insert end:
Insert end:
Insert size (kb): 29.0
Cross references: Insert lengths(kb): 29.0 Gene product: DNA Segment, single copy [D17S122] Alleles: A1, A1, A2, A3, B1, A2, B2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4, 7.6, 7.5, 4.4, 2.6, 1.55, 1.45 (doublet); NotI--9.4 (doublet), 7.6, 1.45; BamHI--11.5, 8.0, 6.6, 5.2, 3.5; EcoRI/BamHI--7.6, 7.5, 5.4, 3.0, 2.7, 2.15, 1.6, 1.45 (doublet); SacII--uncut. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The 7.5 kb EcoRI fragment of the insert has been used to detect the TaqI RFLP at D17S122 and the dosage difference indicative of the duplication mutation associated with Charcot-Marie-Tooth disease (CMT1A). |
| References |
Lupski JR, et al. DNA duplication associated with Charcot-Marie-Tooth disease type 1A. Cell 66: 219-232, 1991. PubMed: 1677316
Pragna I Patel, personal communication
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