| 產(chǎn)品名稱 |
pcDNAI/neo-11B2 |
| 商品貨號(hào) |
B196305 |
| Designations |
pcDNAI/neo-11B2 |
| Species |
Rattus norvegicus, rat |
| Depositors |
CE Gomez-Sanchez, M Zhou |
| Applications |
in another host, produces protein cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase |
| Vector |
Construct size (kb): 8.5 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.5 Gene product: cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase)( Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase), aldosterone synthase, steroid 11-beta/18-hydroxylase, steroid-11-beta-hydroxylase, 11-beta-hydroxylase) [CYP11B2] Target Gene: cytochrome P450, subfamily XIB, polypeptide 2 (steroid-11-beta-hydroxylase, aldosterone synthase) |
| Insert Size (kb) |
1.5 |
| Media |
ATCC® Medium 1235: LB Medium (ATCC medium 1065) with 5 mcg/ml tetracycline and 10 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--7.0, 1.6; BamHI/HindIII--7.7, 0.8; HindIII--7.7, 0.8; PvuII--2.1 (doublet), 1.6 (doublet), 0.8, 0.4; XhoI--7.4, 1.1. The insert contains the following restriction sites (nt from the 5' end): XhoI--539; HindIII--726; HincII--948; PvuII--1039. |
| References |
Zhou M, Gomez-Sanchez CE. Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant. Biochem. Biophys. Res. Commun. 194: 112-117, 1993. PubMed: 8333830
Zimmer WE, et al. Identification of a mouse brain beta-spectrin cDNA and distribution of its mRNA in adult tissues. Brain Res. Bull. 27: 187-193, 1991. PubMed: 1742606
Celso E Gomez-Sanchez, personal communication
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