| Vector |
Construct size (kb): 7.78000020980835 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSELECT-1
Intact vector size: 5.680
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites:
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI PstI SphI HindIII
Construction: pBR322, pGEM-3Zf(+)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): tetR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references: DNA Seq. Acc.: X65334 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.7, 2.1; PvuII--3.1, 2.3, 0.87, 0.8, 0.63, 0.35; HindIII--7.8; BglI--3.7, 2.3, 1.6, 0.2; SphI--7.2, 0.6. Detects genomic EcoRI fragments of the following sizes (kb): human--11.0, 6.1; mouse--18.0. Corresponds to nt 30-2095 of the GenBank record, containing the complete coding sequence (nt 330-1727). |
| References |
Nishi S, et al. Human pancreatic beta-cell glucokinase: cDNA sequence and localization of the polymorphic gene to chromosome 7, band p 13. Diabetologia 35: 743-747, 1992. PubMed: 1511800
Graeme I Bell, personal communication
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