| Vector Information |
Size (kb): 6.4590001106262210 DESCRIPTION OF VECTOR:
Intact vector size: 6.459
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS423, GALL promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): HIS3, ->, 200-1395
terminator: CYC1, ->, 2077-2337
MCS: XhoI...SpeI, ->, 2337-2394
promoter for expression: GALL, <-, 2401-2831
marker(s): ampR, <-, 3982-4912
replicon: 2 micron, ->, 5012-6352 Vector: p423 GALL (plasmid) Promoters: Promoter for expression GALL Construction: pRS423, GALL promoter Marker(s):ampR,HIS3 Construct size (kb): 6.459000110626221 Features: marker(s): HIS3
marker(s): ampR
promoter for expression: GALL
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 2.8, 0.45; EcoRI--6.5; KpnI--5.5, 1.0. The galactokinase (GALL) promoter is a deletion variant of GAL1, which lacks one of the 3 UAS elements required for full induction of the promoter by galactose. The promoter is tightly repressed by glucose and is induced at a moderate level by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
