A shuttle vector which confers both ampicillin and kanamycin resistance in Escherichia coli but only kanamycin resistance in Bacillus subtilis.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Bruckner R, et al. Expression of a chloramphenicol-resistance determinant carried on hybrid plasmids in Gram-positive and Gram-negative bacteria. Gene 32: 151-160, 1984. PubMed: 6442250
