| 產品名稱 | HEK-293.2sus |
|---|---|
| 商品貨號 | B195024 |
| Organism | Homo sapiens, human |
| Tissue | embryonic kidney |
| Cell Type | embryonic |
| Product Format | frozen |
| Morphology | rounded |
| Culture Properties | suspension |
| Biosafety Level | 2 [Contains cells transformed with adenovirus 5 DNA]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | fetus |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | The following is based on the parent cell line (ATCC CRL-1573): This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells. Note: Cytogenetic instability has been reported in the literature for some cell lines. |
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| Derivation | ATCC CRL-1573 (HEK293) cells were progressively adapted from an anchorage-dependent growth mode to a suspension mode. |
| Comments | ATCC CRL-1573 (HEK293) cells were progressively adapted from an anchorage-dependent growth mode to a suspension mode. Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome, it is now clear that only left end sequences are present. The Ad5 insert was cloned and sequenced, and it was determined that a colinear segment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2). |
| Complete Growth Medium | The base medium for this cell line is 293 SFM II (Invitrogen, Catalog No. 11686-029). To make the complete growth medium, add the following component to the base medium: 4mM L-glutamine (final conc.)
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| Subculturing | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Medium renewal: Two to three times weekly |
| Cryopreservation | Freeze medium: Conditioned growth medium (day 3 to 4 cell conditioned medium collected from HEK-293.2sus cultures during subculture procedure) supplemented with 10% (v/v) DMSO. liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X
CSF1PO: 12
D13S317: 12,14
D16S539: 9,13
D5S818: 8
D7S820: 11,12
TH01: 7,9.3
TPOX: 11
vWA: 16,19 |
| Population Doubling Time | approximately 34 hours |
| Name of Depositor | ATCC |
| Year of Origin | October 2008 |
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| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
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| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
