天天操夜夜拍丨国产精品超清白人精品av丨天天爽天天噜在线播放丨国产精品久久久久久久福利竹菊丨色综合天天天天做夜夜夜夜做丨国内自拍xxxx18丨白人と日本人の交わりビデオ丨午夜激情在线观看丨成人18视频丨久久66热人妻偷产精品丨视频一区二区三区在线观看丨操操操插插插丨久久的久久爽亚洲精品aⅴ丨91精品一区二区三区在线观看丨aaa女人18毛片水真多丨99re在线视频精品丨超碰免费在丨中文字幕人妻无码专区app丨午夜香蕉视频丨又大又粗欧美黑人aaaaa片丨久久夫妻视频丨乌克兰性欧美精品高清丨亚洲欧美福利视频丨少妇高潮久久久久久一代女皇丨97国产高清

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Paramecium primaurelia Sonneborn
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
Paramecium primaurelia Sonneborn
Paramecium primaurelia Sonneborn
規格:
貨期:
編號:B194576
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Paramecium primaurelia Sonneborn
商品貨號 B194576
Strain Designations stock 258
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Mexico, 1956
Product Format test tube
Type Strain no
Medium ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Max Temperature: 31.0°C
Min Temperature: 13.0°C
Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCC medium 802 in a 20 x 120 mm test tube.
Subcultivation
Protocol: ATCCNO: 30300 SPEC: This strain is shipped as a growing test tube culture. Upon arrival, remove test tube from sealed plastic envelope, remove plastic seal from cap, and loosen the cap one half turn. Add 1.0 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048 twice weekly. When the tube is filled to within one inch of the top, decant leaving 5.0 ml in the original tube. Subcultures are established by transferring 0.5 ml of a growing culture to 5.0 ml of bacterized ATCC medium 802 in a 20 x 120 mm test tube.
Cryopreservation
Cryoprotective Solution

DMSO ?????????????????????????????????????????????????????????????????????????????????? 1.5 ml

Fresh growth medium w/o bacteria???????????????????????????????? 7.5 ml

MgCl2 (0.5 mM)??????????????????????????????????????????????????????????????????? 0.5 ml

CaCl2 (0.5 mM)??????????????????????????????????????????????????????????????????? 0.5 ml

1.?? Mix the components in the order listed.? Before adding the MgCl2 and the CaCl2 allow the solution to return to room temperature.? When the medium is added to the DMSO the solution will warm up due to chemical heat.

2. ? Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 200 x g for 1 min.

3. Adjust the concentration of cells to 2 x 105/ml in fresh medium.

4.? Mix the cell preparation and the cryoprotective solution in equal portions.

5.? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6.? Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.

7.? Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.

8. To establish a culture from the frozen state add 1.0 ml ATCC medium 802 to the frozen ampule and place it in a 35°C water bath.? Immerse the vial? to a level just above the surface of the frozen material. Do not agitate the vial.

9.   Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate onto the surface of an ATCC medium 919 (non-nutrient agar) plate containing an overlay of 15.0 ml of bacterized ATCC medium 802.

10. Incubate at 25°C.

11. Once the culture is established, transfer 0.5 ml to 5.0 ml of bacterized ATCC medium 802.

12. Follow the protocol for maintenance of culture.
Name of Depositor TM Sonneborn
Year of Origin 1956
Cross References

Nucleotide (GenBank) : U00597 Herpetomonas megaseliae kinetoplast clone 1.1 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00613 Herpetomonas megaseliae kinetoplast clone 1.4 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00618 Herpetomonas megaseliae kinetoplast clone 1.5 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00627 Herpetomonas megaseliae kinetoplast clone 1.6 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00632 Herpetomonas megaseliae kinetoplast clone 1.7 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00634 Herpetomonas megaseliae kinetoplast clone 1.8 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00598 Herpetomonas megaseliae kinetoplast clone 1.10 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00600 Herpetomonas megaseliae kinetoplast clone 1.13 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00601 Herpetomonas megaseliae kinetoplast clone 1.14 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00602 Herpetomonas megaseliae kinetoplast clone 1.16 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00603 Herpetomonas megaseliae kinetoplast clone 1.17 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00604 Herpetomonas megaseliae kinetoplast clone 1.19 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00605 Herpetomonas megaseliae kinetoplast clone 1.21 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00606 Herpetomonas megaseliae kinetoplast clone 1.30 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00607 Herpetomonas megaseliae kinetoplast clone 1.31 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00608 Herpetomonas megaseliae kinetoplast clone 1.32 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00609 Herpetomonas megaseliae kinetoplast clone 1.37 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00610 Herpetomonas megaseliae kinetoplast clone 1.36 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00611 Herpetomonas megaseliae ATCC 30709 kinetoplast partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00612 Herpetomonas megaseliae kinetoplast clone 1.39 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00614 Herpetomonas megaseliae kinetoplast clone 1.43 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00615 Herpetomonas megaseliae kinetoplast clone 1.44 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00616 Herpetomonas megaseliae kinetoplast clone 1.46 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00617 Herpetomonas megaseliae kinetoplast clone 1.48 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00622 Herpetomonas megaseliae kinetoplast clone 1.51 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00623 Herpetomonas megaseliae kinetoplast clone 1.52 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00624 Herpetomonas megaseliae kinetoplast clone 1.53 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00625 Herpetomonas megaseliae kinetoplast clone 1.57 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00626 Herpetomonas megaseliae kinetoplast clone 1.59 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00628 Herpetomonas megaseliae kinetoplast clone 1.60 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00629 Herpetomonas megaseliae kinetoplast clone 1.61 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00630 Herpetomonas megaseliae kinetoplast clone 1.64 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00631 Herpetomonas megaseliae kinetoplast clone 1.68 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00633 Herpetomonas megaseliae kinetoplast clone 1.70 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00661 Herpetomonas megaseliae kinetoplast clone 1.20 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

Nucleotide (GenBank) : U00599 Herpetomonas megaseliae kinetoplast clone 1.100 partially edited cytochrome oxidase subunit III (COIII) RNA sequence.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
朝鲜女人大白屁股ass孕交| 一级黄片毛片| 国产精品极品在线拍| 91欧美一区| 人人妻人人爽人人澡av| 国产xxxxxxxxx| 欧美狠狠爱| 国产二区视频在线观看| 中文字幕人妻被公上司喝醉在线| 中国极品少妇xxxx做受| 日韩和欧美一区二区三区| 日韩精品―中文字幕| videosgratis极品另类灌满高清资源| 色峰视频| 美女黄色毛片| 国产精品久久毛片av大全日韩| 亚洲男人在线天堂| 亚洲免费视频在线观看| 99在线精品视频免费观看20| 亚洲一区二区视频在线观看| 日韩av在线观看免费| 国产美女明星三级做爰| 成人性生交大片免费看r老牛网站| 免费午夜av| 叶山小百合av一区二区| 色婷婷激情综合| 精品成人乱色一区二区| 91精品又粗又猛又爽| 国产丝袜一区二区在线| 丰满少妇被猛烈进入无码| 亚洲中文久久精品无码| 成人精品免费视频在线观看 | 国产人伦视频| 免费久久日韩aaaaa大片| 亚洲精品成人网久久久久久| 国产在线观看无码免费视频| 亚洲日韩乱码中文无码蜜桃臀网站| 国产日韩精品视频无码| 国产福利小视频在线| 精品国产91乱码一区二区三区| 亚洲欧美日韩成人在线 | 亚洲欧美在线综合| 亚洲视频免费观看| 成人三级黄色片| 亚洲高清中文字幕| 欧美裸体性生活| 无码人妻人妻经典| 国产精品高潮呻| 中文字幕免费高| 日本欧美一级aaaaa毛片| 无码中文字幕av免费放dvd | 91国内产香蕉| 天天操天天拍| 福利一区二区在线| 亚洲第二页| 一区二区三区av波多野结衣 | 麻豆一区二区在线| 亚洲精品国产美女久久久| 又大又粗又长的高潮视频| 天堂网ww| 久久婷婷丁香七月色综合| 欧美中文字幕在线| 黄色毛片网站| 久久精品人人做人人爽电影| 久草在线最新视频| 婷婷综合久久| 国产精品资源在线观看 | 精品无码国产自产拍在线观看| 国产一级做a爱片在线看免| 欧美色爱综合网| jizz亚洲女人高潮大叫| 一区二区三区免费看| 国产免费网址| av在线入口| 中文字幕无码日韩av| 青青操视频在线观看| 久久久精品久| 中文字幕av一区二区三区高| 国产边摸边吃奶叫床视频| 在线观看国产小视频| 亚洲中又文字幕精品av| 嫩草午夜少妇在线影视| 欧美性猛交乱大交xxxx| 成人comx8| 无码熟熟妇丰满人妻啪啪| 又大又黄又粗高潮免费| 国产盗摄一区二区| 围产精品久久久久久久| 亚洲精品揄拍自拍首页一| 精品白嫩bbwbbwbbw韩国| 香港台湾经典三级a视频| 喷水白丝蜜臀av久久av| 成人免费黄| 天堂久久网| 亚洲天堂午夜| 中文字幕无码肉感爆乳在线| 亚洲欧美中文字幕在线一区| 四川50岁熟妇大白屁股真爽| 日韩久久一区| 国产成人无码免费视频麻豆| 91av在线播放视频| 狠狠爱天天综合色欲网| 国产高清乱码女大生av| 激情黄色av| 亚洲欧美色图片| 免费黄色一级| 国产美女网| 国产亚洲精品久久久久久青梅| 国产精品视频a| 成人免费视频高潮潮喷无码| 亚洲毛片一区二区三区| wwwxx欧美| 亚欧av在线| 99精品福利| v天堂中文在线| 亚洲在线免费观看视频| 自拍偷拍欧美| 亚洲永久精品ww.7491进入| 国产在线精品观看| 五月综合激情婷婷六月色窝| 婷婷综合基地俺也来| 欧美一本| 色视频久久| 九一九色国产| 成人麻豆日韩在无码视频| 亚洲最大av资源网在线观看| 97caop| 亚洲日本欧美| 久久久激情网| 欧美a大片| 在线观看亚洲国产| 艳妇臀荡乳欲伦交换在线播放| 亚洲欧美日韩成人高清在线一区| 一区二区三区中文字幕在线| 香蕉影音| 欧美高清处破的免费视频| 无码无遮挡又大又爽又黄的视频 | 亚洲高清视频在线播放| 女同一区| 日韩精品久久久免费观看| 台湾成人毛片| 99mav| 日本高清免费毛片久久| 你懂的欧美| 对白脏话肉麻粗话av| 亚洲精品成a人在线观看| 无码人妻出轨黑人中文字幕| 极品videosvideo喷水| 国产中文在线观看| 欧美成人xxxxx| 好男人社区影院www| 少妇xxxx| 肉视频在线观看| 少妇人妻无码永久免费视频| 最近中文字幕免费视频| 国产一级视频在线播放| 毛片免费视频肛交颜射免费视频| 可以直接观看的av| 99久久99精品久久久久久| 天天撸在线视频| 久久久久9| 高清成人免费视频| 色图自拍| 免费黄色av| 国产精品久久成人| 激情久久久久久久| 精品深夜av无码一区二区| 亚洲人网站| 在线观看成人动漫| 日.本人xxxxxxxxx19| 美女av一区二区三区| 激情内射亚洲一区二区三区爱妻| 乌克兰少妇xxxx做受| 最近中文字幕mv| 欧美激情视频一区二区| 日韩av色图| 又长又硬又粗一区二区三区| 99久久国产成人免费网站| 妺妺窝人体色www婷婷| 在线日韩av永久免费观看| 又色又爽又黄又免费的照片| 日本熟妇乱人伦xxxx| 久久亚洲欧美日本精品| 少妇超碰| 人妻少妇精品中文字幕av蜜桃| 少妇媚药按摩中文字幕| 久久久久久久国产| 成人亚洲一区二区三区在线| 欧美一区二区高清视频| 国产亚洲人成无码网在线观看| 日本女人一级片| 暖暖日本在线观看免费| 澳门一级黄色片| caoprom在线| 麻婆豆传媒一区二区三| 国产黄色网页| 奇米色欧美一区二区三区| 欧美亚洲精品一区二区在线观看 | 成人高清视频在线| 亚洲色图13p| caopeng在线视频| wwww日本60| 日本熟妇乱子伦xxxx| 最近中文字幕| 五月激情丁香婷婷| 国产午夜精品一区二区三区老| 精品日本一区二区三区在线观看| 国产精品视频分类| 九九在线观看高清免费| 波多野吉衣一区| 天堂国产| 国产成人拍精品视频午夜网站| 欧美三日本三级少妇99印度| 国内三级在线| 亚洲欧美18岁网站| 亚洲天堂网一区二区| 国产精品va在线观看无码| 国产男女在线| 国产吴梦梦无套系列| 成人国产一区二区三区精品麻豆| av噜噜噜在线播放| 视频一区 视频二区 视频三区 视频四区 国产 | 亚洲不卡一卡2卡三卡4卡5卡| 男人撕开奶罩揉吮奶头视频| 性欧美在线视频| 亚洲国产精品女人久久久| 99精品偷自拍| 在线日韩精品视频| 国产粉嫩一区二区三区| 国产色婷婷亚洲999精品小说| 狠狠爱亚洲综合久久| 国产丝袜网站| 亚洲精品国产suv一区| 成人亚洲区无码区在线点播| 性做久久久久久免费观看| 国产精品毛片久久久久久| 68精品久久久久久欧美| 色妞综合网| 亚洲精品99久久久久久欧美版| 亚洲国产爱| 999热视频| www.久久视频| 国产自偷亚洲精品页65页| 黄色录像一级大片| 久久亚洲精品无码观看不| 香港aa三级久久三级| 国产精品福利视频| 亚洲欧洲成人精品av97| 另类综合小说| 午夜av在线播放| 99在线观看免费视频| 天天澡夜夜澡狠狠久久| 韩国和日本免费不卡在线v| 五月天爱爱| 日本鲜嫩鲜嫩bbw| 无码精品久久久久久人妻中字| 国产sm在线| 91中文字幕| 亚洲精品一区二区三区福利| 亚洲综合网国产精品一区| 久久久久久久久免费视频| 久久久无码人妻精品无码| 三上悠亚ssⅰn939无码播放| 欧美极品在线视频| 加勒比无码一区二区三区| 久久精品欧美一区二区| 超污视频在线观看| 欧美一区二区三区视频在线| 538精品一线| 高清成人免费视频| 免费看黄色av| 欧美亚洲熟妇一区二区三区| 成在人av抽搐高潮喷水流白浆| 久久靖品| 黄色毛片一级片| 91久久精品日日躁夜夜欧美| av一区免费| 丰满少妇乱子伦精品看片| 欧美性一区二区| 欧美熟妇另类久久久久久不卡| 国产成人啪精品视频免费软件 | 爱情岛亚洲论坛福利站| 成人内射国产免费观看| 超碰中文在线| 久久免费国产视频| 亚洲欧美日韩中文加勒比| 少妇高潮久久久久久一代女皇| a级免费毛片| 手机国产乱子伦精品视频 | 日韩欧美一级大片| 亚洲国产久| 欧美亚洲综合另类色妞网| 欧美精品乱码久久久久久按摩| 国内精品久久久久影视老司机| 久久激情小说| 欧美视频xxx| 夫妻一区二区| 欧美aa级| 日韩精品国产另类专区| 天堂av资源网| 美女诱惑av| 夜夜躁狠狠躁日日躁2021日韩| 欧美暧暧视频| 久久论理| 成年黄色网| 国产精品人妻一区二区高| 蜜桃麻豆www久久囤产精品| 中文免费视频| 高清自拍亚洲精品二区| 最新国产黄色网址| 超清无码av最大网站| 免费看欧美一级片| 天堂网资源| 亚洲国产精品va在线观看香蕉| 西西人体自慰扒开下部93| 青青草视频偷拍| 九色视频在线免费观看| 日韩一区二区三区无码影院| 国产精品自产拍在线观看免费| 啪啪免费小视频| 尤物精品国产第一福利网站| 久久偷偷| 中国真实的国产乱xxxx| 国产亚洲精品久久久久秋霞不卡| 91蝌蚪| 91久久亚洲| 天天噜噜噜噜噜噜| 91精品无人区麻豆乱码1区2区介绍| 久久人人97超碰a片精品| 青青草无码伊人久久| 老女人人体欣赏a√s| 亚洲女初尝黑人巨高清| 国内精品久久久久影院免费| 日本xxxx高潮少妇| 97偷拍视频| 欧美在线黄| 极品气质女神呻吟娇喘91| 久久久综合亚洲91久久98| 国内精品久久久久久久久久久| 中文字幕视频在线|