| Insert |
DNA: cDNA Insert lengths(kb): 1.9 Tissue: Hs578T cell line Gene product: fibronectin 1 [FN1] Alleles: A2, E2, A1, B2, D2, B1, D1, E1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): PvuII--4.8, 0.6; HindIII--5.6; BamHI--4.5, 0.7; HindIII/BamHI--3.0, 1.5, 0.7; EcoRI--3.6, 1.5. A 0.5 kb HindIII/BamHI fragment has been used to detect the TaqI polymorphism. The 2.25/2.2 kb TaqI RFLP can be resolved on 1% agarose gels run for 20 cm. Enzyme(s) not detecting polymorphism: HaeIII, HindIII, EcoRI, PstI, BglII. |
| References |
Kornblihtt AR, et al. Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. EMBO J. 4: 1755-1759, 1985. PubMed: 2992939
Gardella R, et al. A new TaqI RFLP of the human fibronectin (FN1) gene. Nucleic Acids Res. 17: 10520, 1989. PubMed: 2574858
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